Within the  framework of “In situ Conservation of Plant Genetic Diversity in Turkey Project”, genetic diversity of Anatolian black pine populations from Kazdağı was investigated by seedling traits. Cones were collected from 315 trees by random sampling.  Then, seeds were sown in a nursery by complete randomized design as 3 replicates. Seedlings were investigated for two years for 8 seedling characteristics. 

      Variation between populations were observed for only date of bud set in 1996 trait. Families were different for growth traits. Moreover family heritabilities were not significant due to variation within families. Genetic distances estimated for the populations showed that populations are not differentiated significantly. 

      As a result Eybekli, Mıhlıdere and Gürgendağ populations were recommended as “Gene Management Zone - GMZ”. 

Key Words: Pinus nigra, genetic diversity, heritability, GMZ.